Morphological Validation of the Alternative Allograft Use in Ophthalmic Surgery

    

    Abstract

    Morphological Validation of the Alternative Allograft Use in Ophthalmic Surgery

    O.R. Shangina, L.A. Musina, R.A. Khasanov

    Russian Center foe Eye and Plastic Surgery, Ufa, Russian Federation

    Purpose. Morphological assessment of fibrearchtechtonics of dispersed allografts made of cadaveric fascia lata.

    Material and methods. The morphological study of cadaveric fascia lata, made of its whole spirits-preserved and crushed grafts (10 samples each) was carried out. Light microscopy of collagen fibers (thickness of fiber bundles and gaps between them) in glycosaminoglycans (Hale's hystochemical reaction) was performed.

    Results. Studies of fascia lata samples which underwent technological processing showed that the tissue structure and its glycosaminoglycans are preserved; there was only a slight defibration of collagen fiber bundles. At the same time cellullar elements undergo complete destruction which reduces the immugenicity of allograft. It has been established that, in general, dispersed allografts have the same properties as native and preserved ones.

    Conclusion. Physicochemical processing of donor tissues, its crushing, fractioning and radiant sterilization allow to keep fibrearchtechtonics of fascia lata when making dispersed allografts, what is important for regenerative processes in ophthalmic surgery.

    Key words: morphological study, fibrearchtechtonics, fascia lata, structure, grafts, injectable biomaterials

    

    For quoting: Shangina O.R., Musina L.A., Khasanov R.A. Morphological Validation of the Alternative Allograft Use in Ophthalmic Surgery.Point of view. East – West. 2023;3: 22–26. DOI: https://doi.org/10.25276/2410-1257-2023-3-22-26

    Corresponding author: Olga R. Shangina, alloolga@mail.ru